The group trend of lanthanides binding to DNA and DNAzymes with a complex but symmetric pattern.
نویسندگان
چکیده
Using sensitized Tb(3+) luminescence spectroscopy as a tool, binding of 14 lanthanides to a lanthanide-dependent DNAzyme is studied, where the binding affinity is symmetric across the series and the tightest binding occurs with Nd(3+) and Ho(3+). This trend does not correlate with DNAzyme activity, suggesting that metal binding may not be the rate-limiting step of the DNAzyme catalysis.
منابع مشابه
BINDING OF THE ANTITUMOR DRUG ADRIAMYCIN TO DNA-HISTONE COMPLEXES
Isotherms of the binding of the anthracycIine antibiotic, adriamycin (adriblastin), to DNA histone complexes was studied by means of spectroscopic analysis. The results indicated that: (a) binding of adriamycin to histones reduced the interaction of histones with DNA, (b) binding of the drug to DNA did not change the binding affinity of histone to DNA and, (c) in the explored binding range...
متن کاملIn vitro selection of a new lanthanide-dependent DNAzyme for ratiometric sensing lanthanides.
Developing biosensors for lanthanides is an important but challenging analytical task. To address this problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a region of 35 random nucleotides in the presence of Lu(3+), since Lu(3+) was reported to be the most efficient lanthanide for RNA cleavage. The resulting DNA sequences can be aligned to a single fa...
متن کاملA spectroscopic study on Calf thymus DNA binding properties of nickel (II) complex with imidazole derivatives of 1,10-phenanthroline ligand
In this study, a nickel (II) complex with 1,10-phenanthroline based ligand, [Ni(FIP)2](OAC)2 (1) with FIP = 2-(Furan-2-yl)-1H-Imidazole[4,5-f][1,10] phenanthroline as ligand was synthesized and characterized by spectroscopic methods and elemental analysis. The interaction of [Ni(FIP)2](OAC)2 (1) with calf-thymus DNA (ct-DNA) was studied by UV-vis absorption, fluorescence spectroscopies and visc...
متن کاملEffects of Vitamin K3 on Cytotoxicity and DNA-binding Properties of a Palladium(II) Complex as a Potential Antitumor Agent
In many diseases such as cancer, simultaneous use of two or more pharmacologically active agents will be more effective and have fewer side effects. In this study a new palladium(II) complex with formula of [Pd(phen)(py-dtc)]NO3 (where is phen 1,10-phenanthroline and py-dtc is n-propyldithiocarbamate), was synthesized. The cytotoxic activity of this complex was tested against leukemia K562 cell...
متن کاملDetermination of the Binding Constant of Terbium-Transferrin
Apotransferrin (apo Tf) in 0.1 M N-(2hydroxyethyl)piperazine-N2-ethanesulfanic acid at 25 ˚C and pH 7.4 has been titrated with acidic solution of Tb3+. The binding of Tb3+ at the two specific metal-binding sites of transferrin was followed from the changes in the difference UV spectra at 245 nm. The molar absorptivity per binding site for Tb3+...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 50 80 شماره
صفحات -
تاریخ انتشار 2014